Outbreak of bloodstream infection caused by Burkholderia cepacia complex: the role of genetic sequencing in investigation.
IRAS (infecções relacionadas à assistência à saúde) or hospital infections are one of the greatest challenges of the 21st century. According to data from the World Health Organization (WHO), of all patients operated in the world, one million people die from hospital infections and seven million have postoperative complications.
Among the causes of hospital infections are lack of hands hygiene, indiscriminate use of antibiotics, breakdown of care protocols and environmental contamination.
The Neobiome HealthCare platform is a tool for healthcare professionals, who work with infection control, for fast decision-making based on the knowledge of the hospital microbiome and control of hospital processes.
With the tools available on the platform, it is possible to map out flaws in processes, validate sanitation procedures, identify reservoirs of microbiological contaminations in the environment, infer potential routes of cross contamination, promote educational actions, and perform sterility tests on materials.
Hospital Alemão Oswaldo Cruz, one of the best and most modern hospitals in Brazil with 354 beds, located in São Paulo.
The hospital had an outbreak caused by the bacterium Burkholderia cepacia complex, an important pathogen associated with nosocomial outbreaks. Between March 2012 and July 2014, 12 patients were identified with infections in the bloodstream in different sectors of the hospital.
Visits to the operating area revealed possible problems related to the process of storage and distribution of injectable and other thermolabile drugs. For the accurate and visual identification of the problem, environmental samples were collected from points of this process to perform analysis through culture and digital microbiological diagnosis.
Digital microbiological diagnosis has proved to be a promising tool for investigating outbreaks in hospitals. With this method it was possible to identify the presence of Burkholderia cepacia species in the pharmacy refrigerator, in the coolbox and in reusable ice packs, while all samples analyzed by culture were negative. With this information it was possible to take several actions of control such as intensification of hygiene routines, acquisition of easily washable refrigerators and use of Gelo-x (A reusable ice bag).